Detection of Leptospira spp. in the semen and urine of bulls serologically reactive to Leptospira interrogans serovar hardjo
نویسندگان
چکیده
منابع مشابه
DNA probe for detection of the Leptospira interrogans serovar hardjo genotype hardjo-bovis.
A DNA probe is described for the diagnostic and taxonomic identification of the North American cattle pathogen Leptospira interrogans genotype hardjo-bovis. The probe is specific for this genotype and does not hybridize to genomic DNA of any other leptospire pathogen commonly found in North America.
متن کاملMolecular detection of Leptospira spp. in the urine of cattle in northern Iran
Leptospirosis is a zoonosis of worldwide distribution, caused by Leptospira interrogans and is considered as an emerging global public health problem. Transmission usually results from direct or indirect exposure to the urine or other body fluids of leptospiruric animals which may become a source of infection for human or other animals. Having a humid climate with plenty of annual rainfall, Gui...
متن کاملIdentification and characterization of the protein antigens of Leptospira interrogans serovar hardjo.
We radiolabeled Leptospira proteins with [35S]methionine. Solubilized extracts of radiolabeled L. interrogans serovar hardjo strain hardjoprajitno were analyzed by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. We compared the protein profile obtained in this manner to the protein profiles of various [35S]methionine-labeled Leptospira spp. The profil...
متن کاملAddition of rabbit serum to EMJH medium improves isolation of Leptospira interrogans serovar hardjo.
The addition of 2% pooled rabbit serum to semi-solid commercial EMJH medium with EMJH enrichment and 0,5 mg of 5-fluorouracil per ml was found to enhance the growth rate and success of isolation of Leptospira interrogans serovar hardjo from bovine urine. Cultures made on media without serum had to be kept for more than 130 days before being discarded as negative.
متن کاملWhole Genome Sequencing Allows Better Understanding of the Evolutionary History of Leptospira interrogans Serovar Hardjo
The genome of a laboratory-adapted strain of Leptospira interrogans serovar Hardjo was sequenced and analyzed. Comparison of the sequenced genome with that recently published for a field isolate of the same serovar revealed relatively high sequence conservation at the nucleotide level, despite the different biological background of both samples. Conversely, comparison of both serovar Hardjo gen...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2005
ISSN: 1517-8382
DOI: 10.1590/s1517-83822005000100009